Observation of a conformational effect in peptide molecule by reversed-phase high-performance liquid chromatography.
نویسنده
چکیده
RP-HPLC of analogues of oxytocin containing a reduced peptide bond was studied using various columns and buffers. Anomalous behavior of one analogue served as a basis for the discussion of possible conformational consequences of this substitution.
منابع مشابه
Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column
The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...
متن کاملSeparation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column
The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...
متن کاملReversed-phase Salt Assisted Liquid-liquid Extraction: A New Technique for Preconcentration and Determination of Crocin in Herbal Medicines by HPLC
A reversed-phase salt assisted liquid-liquid extraction (RP-SALLE) method was developed for the preconcentration and determination of trace crocin in herbal medicines by high-performance liquid chromatography (HPLC). In developed method, the roles of the aqueous and organic phases are inverted so that the analyte is extracted from the organic into the aqueous phase after salt addition and phase...
متن کاملSimulataneous Analysis of Trimethoprim and Sulphamethoxazole Drug Combinations in Dosage Forms by High Performance Liquid Chromatography
A sensitive high performance liquid chromathography (HPLC) analytical procedure was developed for the quantitive determination of trimethoprim (TM* and sulphamethoxazole (SM) in commercial dosage forms. C18 analytical column (stainless steel, 25 cm * 4.6 mm i.d.) was packed with 5-*m particles of the reversed phase material and used for assays. Mobile phase containing 0.025 M sodium phosphate...
متن کاملA simple, sensitive and rapid isocratic reversed-phase high-performance liquid chromatography method for determination and stability study of curcumin in pharmaceutical samples
Objective: This study was designed to develop and validate a new reversed-phase high-performance liquid chromatography (RP-HPLC) method based on Q2 (R1) International Conference on Harmonization (ICH) guideline for determination of curcumin in pharmaceutical samples. Materials and Methods: The HPLC instrument method was optimized with isocratic elution with acetonitrile: ammonium acetate (45:55...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of chromatography
دوره 644 2 شماره
صفحات -
تاریخ انتشار 1993